GROUNDNUT SUBSTITUTE MEDIA FOR BACTERIOLOGICAL CULTURE

CORRESPONDING AUTHOR:
Dr. Hemina Desai,
#40, RajdhaniBunglows,
Near RamwadiTekra,
Isanpur Road,
Ahmedabad-382443.
Email: hemina@ymail.com

ABSTRACT:  BACKGROUND:  In the developing countries manual methods are still used for the cultivation of the bacteria and many a times difficulties are involved in procuring  suitable components for preparation of various nutrient media.  The need has therefore arisen , to investigate the problem of exploitation of indigenous material such as groundnuts to substitute  commercial preparations used in the various nutrient media for bacteriological culture.  AIMS & OBJECTIVES:

In the present study , we investigated the problem of incorporation of groundnut as  an indigenous protein source, to substitute the use of commercially available nutrients in various simple and selective media.  SETTINGS & DESIGN: We have used manual methods for preparation of the culture media using both groundnut powder and commercial preparations as a source of protein. Further  the various bacteriological parameters were studied comparing the both.  MATERIAL & METHODS: In present study, the raw groundnut powder was used in the preparation of various media and these media have been further tested for various parameters like – bacterial growth ,  motility, as a medium for maintenance of stock cultures and for carrying out antibiotic sensitivity tests.  RESULTS & CONCLUSIONS: The results of groundnut substitute media for bacteriological culture  was comparable to that of other commercially available media and it can be employed for routine bacteriological testing of the clinical samples.

KEYWORDS: groundnuts, bacteriological culture ; substitute media