Role of Endothelial Nitric Oxide Synthase (T786C) Gene Polymorphism in the Development of Coronary Artery Disease.

Corresponding Author:
Dr. Namrata Bhutani,
D-162, Tagore Garden Extn.,
New Delhi-110027,
India.
E-mail: namrata.bhutani@gmail.com

ABSTRACT

BACKGROUND

Recent studies suggest a cause and effect relationship between eNOS gene polymorphism and increased incidence of insulin resistance as NO deficiency is associated with decreased vasodilation and altered binding of insulin to insulin receptor. Current studies suggest a worldwide association between eNOS-786T/C gene polymorphism and genetic susceptibility to insulin resistance. We wanted to study eNOS -786T/C gene polymorphism, nitric oxide and insulin Resistance in patients with CAD in Indian population.

METHODS

This study consisted of 60 cases who were adult patients with documented coronary artery disease. 60 age and sex matched healthy subjects were selected as controls. In these groups, fasting serum insulin was measured by ELISA, fasting serum nitric oxide by modified Griess reaction and fasting plasma glucose on fully automated chemistry analyser (Hitachi 902). Furthermore, HOMA-IR was calculated mathematically. The eNOS gene loci was amplified by using PCR and by RFLP. A p-value <0.05 was considered significant. Statistical analysis was performed with  SPSS.

RESULTS

The mean serum NO levels were very significantly lower in case groups and mean HOMA-IR levels in the study group were significantly higher as compared to the control group (p=0.000) suggesting the role of insulin resistance in CAD. The frequency of Tallele was 76.67% in the study group and 81.67% in the control group while the frequency of Callele was 23.33% in the study group and 18.33% in the control group respectively. This difference was found to be statistically significant (p=0.0123).

CONCLUSIONS

Endothelial Nitric Oxide Synthase (T786C) gene polymorphism, is significantly associated with occurrence of coronary artery disease. Thus, this polymorphism could be a potential risk factor for development of coronary artery disease in adult Indian population.

KEY WORDS

Coronary Artery Disease, Nitric Oxide, Insulin Resistance, Polymorphism, RFLP