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2016 Month : September Volume : 5 Issue : 71 Page : 5190-5195

IMMUNOHISTOCHEMICAL AND POLYMERASE CHAIN REACTION STUDY ON EXPRESSION OF HPV AND HSV AND ITS HISTOPATHOLOGICAL PATTERN CORRELATION IN CERVICAL LESIONS.

Smita Chouhan1, Reeni Malik2, Rajendra Kumar Nigam3, Pramila Jain4

Corresponding Author:
Dr. Smita Chouhan,
C/o. Mr. N. S. Chouhan,
#40, Shukla Colony,
Shantiniketan,
Mandsaur-458001, M. P.
E-mail: smitachouhan84@gmail.com

ABSTRACT

BACKGROUND

Cervical cancer has a major impact on developing countries, where screening programmes are not well established or effective.

AIM

This study aims to detect HPV and HSV in cervical lesions using Immunohistochemistry and Polymerase chain reaction.

SETTINGS AND DESIGN

85 biopsy samples were obtained from out and inpatients attending Gynaecology Department in Gandhi Medical College and associated hospital, Bhopal between January - August 2015.

MATERIALS AND METHODS

Two biopsy specimens of the cervix were simultaneously collected from the same patient; one for histopathological examination and other for polymerase chain reaction. The samples were graded as chronic cervicitis, Cervical Intraepithelial Neoplasia (CIN) I, II, III, and Squamous Cell Carcinoma (SCC) and cervical adenocarcinoma by pathologist in Department of Pathology.

RESULTS

Out of 85 cervical biopsies, HPV (IHC) positivity was maximum for chronic cervicitis with dysplasia (77.8%). IHC using HSV in cervical lesions showed 45.2% positivity in chronic cervicitis; 44.4% in chronic cervicitis with dysplasia and 21.9% in squamous cell carcinoma.

CONCLUSION

This study reveals a significant detection of HPV in the individuals with pre-cancerous lesions by the use of advanced techniques such as IHC and PCR.

KEYWORDS

Cervical Intraepithelial Neoplasia, Human Papillomavirus, Squamous Cell Carcinoma, Immunohistochemistry, Polymerase Chain Reaction.

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